rabbit anti phosphorylated p akt (Cell Signaling Technology Inc)
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Rabbit Anti Phosphorylated P Akt, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+phosphorylated+p+akt/pm41763584-177-36-77
Average 86 stars, based on 1 article reviews
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Incubation:Article Title: miR-126-5p regulates H9c2 cell proliferation and apoptosis under hypoxic conditions by targeting IL-17A Article Snippet: The membranes were blocked with 5% non-fat milk in TBST (containing 0.1% Tween-20; cat. no. T1081-500; Beijing Solarbio Science & Technology Co., Ltd.) for 50 min at room temperature. .. Subsequently, the membranes were incubated at 4 ̊C overnight with the following primary antibodies: Rabbit anti-IL-17A (1:1,000; cat. no. ab136668; Abcam), rabbit anti-B-cell lymphoma 2 (Bcl-2; 1:1,000; cat. no. ab32124; Abcam), rabbit anti-AKT (1:500; cat. no. ab8805; Abcam), Article Title: Edaravone dexborneol promotes oligodendrocyte precursor cell proliferation and differentiation to repair white matter and improve long-term outcomes after ischemic stroke. Article Snippet: Then, 25 μg of protein was subjected to electrophoresis using 10–12% SDS polyacrylamide gels and transferred to polyvinylidene difluoride (PVDF) membranes (cat. no. 10600021; GE Healthcare). .. The membranes were blocked with 10% non-fat milk in Tween/Tris-buffered salt solution (20 mM Tris-Cl pH 7.5, 0.15 M NaCl, 0.05% Tween-20) for 1 h, then incubated overnight at 4̊C with rabbit anti-Akt (cat. no. 4691), Article Title: Ginkgolide B inhibits hydrogen peroxide-induced apoptosis and attenuates cytotoxicity via activating the PI3K/Akt/mTOR signaling pathway in H9c2 cells Article Snippet: .. The membranes were blocked with 5% BSA (Gibco; Thermo Fisher Scientific, Inc.) at room temperature for 2 h and incubated overnight at 4°C with primary antibodies against rabbit anti-Bax (1:1,000; cat. no. 2772), rabbit anti-Bcl-2 (1:1,000; cat. no. 2870), rabbit anti-cleaved caspase-3 (1:1,000; cat. no. 9664), rabbit anti-caspase-3 (1:1,000; cat. no. 9662), rabbit anti-Akt (1:1,000; cat. no. 9272), Article Title: Anterior thalamic nuclei deep brain stimulation inhibits mossy fiber sprouting via 3′,5′-cyclic adenosine monophosphate/protein kinase A signaling pathway in a chronic epileptic monkey model Article Snippet: Total protein (60 μg) was resolved by SDS-polyacrylamide gel electrophoresis on a 12% polyacrylamide gel and transferred to polyvinylidene difluoride membrane (Millipore, Billerica, MA, USA). .. The membranes were blocked with 10% milk for 1 h and then incubated with the following primary antibodies: rabbit anti-β-actin (1:5000; Sigma-Aldrich, St. Louis, MO, USA, A5060), other:Article Title: miR-126-5p regulates H9c2 cell proliferation and apoptosis under hypoxic conditions by targeting IL-17A Article Snippet: Proteins (10 μg/lane) were separated via 10% SDS-PAGE (cat. no. P1200-25T; Beijing Solarbio Science & Technology Co., Ltd.) and transferred to PVDF membranes (Beijing Solarbio Science & Technology Co., Ltd.). Article Title: Knockdown of ETV4 promotes autophagy-dependent apoptosis in GBM cells by reducing the transcriptional activation of EMP1 Article Snippet: Protein bands were visualized using SuperSignal West Pico Plus (Thermo Fisher Scientific, Inc.) and a GS800 densitometer scanner (Bio-Rad Laboratories, Inc.). Cell-Signaling:Article Title: Ginkgolide B inhibits hydrogen peroxide-induced apoptosis and attenuates cytotoxicity via activating the PI3K/Akt/mTOR signaling pathway in H9c2 cells Article Snippet: .. The membranes were blocked with 5% BSA (Gibco; Thermo Fisher Scientific, Inc.) at room temperature for 2 h and incubated overnight at 4°C with primary antibodies against rabbit anti-Bax (1:1,000; cat. no. 2772), rabbit anti-Bcl-2 (1:1,000; cat. no. 2870), rabbit anti-cleaved caspase-3 (1:1,000; cat. no. 9664), rabbit anti-caspase-3 (1:1,000; cat. no. 9662), rabbit anti-Akt (1:1,000; cat. no. 9272), Article Title: Anterior thalamic nuclei deep brain stimulation inhibits mossy fiber sprouting via 3′,5′-cyclic adenosine monophosphate/protein kinase A signaling pathway in a chronic epileptic monkey model Article Snippet: Total protein (60 μg) was resolved by SDS-polyacrylamide gel electrophoresis on a 12% polyacrylamide gel and transferred to polyvinylidene difluoride membrane (Millipore, Billerica, MA, USA). .. The membranes were blocked with 10% milk for 1 h and then incubated with the following primary antibodies: rabbit anti-β-actin (1:5000; Sigma-Aldrich, St. Louis, MO, USA, A5060), |

