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rabbit anti phosphorylated p akt  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc rabbit anti phosphorylated p akt
    Rabbit Anti Phosphorylated P Akt, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+phosphorylated+p+akt/pm41763584-177-36-77
    Average 86 stars, based on 1 article reviews
    rabbit anti phosphorylated p akt - by Bioz Stars, 2026-11
    86/100 stars

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    Related Articles

    Incubation:

    Article Title: miR-126-5p regulates H9c2 cell proliferation and apoptosis under hypoxic conditions by targeting IL-17A
    Article Snippet: The membranes were blocked with 5% non-fat milk in TBST (containing 0.1% Tween-20; cat. no. T1081-500; Beijing Solarbio Science & Technology Co., Ltd.) for 50 min at room temperature. .. Subsequently, the membranes were incubated at 4 ̊C overnight with the following primary antibodies: Rabbit anti-IL-17A (1:1,000; cat. no. ab136668; Abcam), rabbit anti-B-cell lymphoma 2 (Bcl-2; 1:1,000; cat. no. ab32124; Abcam), rabbit anti-AKT (1:500; cat. no. ab8805; Abcam), rabbit anti-phosphorylated (p)-AKT (1:1,000; cat. no. 4060; Cell Signaling Technology, Inc.), rabbit anti-PI3K (1:1,000; cat. no. ab32089; Abcam), rabbit anti-p-PI3K (1:1,000; cat. no. ab182651; Abcam), rabbit anti-Bcl-2 associated X protein (Bax; 1:1,000; cat. no. ab32503; Abcam), rabbit anti-cleaved caspase-3 (1:500; cat. no. ab13847; Abcam) and rabbit anti-GAPDH (1:2,500; cat. no. ab9485; Abcam). .. After washing with 0.1% TBST, the membranes were incubated with a goat anti-rabbit IgG H&L secondary antibodies (1:1,000; cat. no. ab6940; Abcam) for 50 min at room temperature.

    Article Title: Edaravone dexborneol promotes oligodendrocyte precursor cell proliferation and differentiation to repair white matter and improve long-term outcomes after ischemic stroke.
    Article Snippet: Then, 25 μg of protein was subjected to electrophoresis using 10–12% SDS polyacrylamide gels and transferred to polyvinylidene difluoride (PVDF) membranes (cat. no. 10600021; GE Healthcare). .. The membranes were blocked with 10% non-fat milk in Tween/Tris-buffered salt solution (20 mM Tris-Cl pH 7.5, 0.15 M NaCl, 0.05% Tween-20) for 1 h, then incubated overnight at 4̊C with rabbit anti-Akt (cat. no. 4691), rabbit anti-phosphorylated (p-)Akt (Thr308; cat. no. 13038), rabbit anti-mTOR (cat. no. 2983), rabbit anti-p-mTOR (Ser2481; cat. no. 2974), rabbit anti-S6 ribosomal protein (cat. no. 2217), rabbit anti-p-S6 (Ser235/236; cat. no. 4858) and rabbit anti-GAPDH (cat. no. 2118) primary antibodies (all from CST Biological Reagents Co., Ltd. and used at a dilution of 1:1000). .. After washing for 30 min, the PVDF membranes were incubated with the horseradish peroxidase-conjugated anti-rabbit IgG secondary antibody (Abcam; cat. no. ab6721; 1:10,000) at room temperature for 1.5 h. Reactivity was visualized using an enhanced chemiluminescence (ECL) kit (MilliporeSigma) and captured with a Bio-Rad ECL detection system.

    Article Title: Ginkgolide B inhibits hydrogen peroxide-induced apoptosis and attenuates cytotoxicity via activating the PI3K/Akt/mTOR signaling pathway in H9c2 cells
    Article Snippet: .. The membranes were blocked with 5% BSA (Gibco; Thermo Fisher Scientific, Inc.) at room temperature for 2 h and incubated overnight at 4°C with primary antibodies against rabbit anti-Bax (1:1,000; cat. no. 2772), rabbit anti-Bcl-2 (1:1,000; cat. no. 2870), rabbit anti-cleaved caspase-3 (1:1,000; cat. no. 9664), rabbit anti-caspase-3 (1:1,000; cat. no. 9662), rabbit anti-Akt (1:1,000; cat. no. 9272), rabbit anti-phosphorylated (p)-Akt (p-Akt; 1:1,000; cat. no. 4060), rabbit anti-mTOR (1:1,000; cat. no. 2983), rabbit anti-p-mTOR (p-mTOR; 1:1,000; cat. no. 5536), and rabbit anti-GAPDH (1:1,000; cat. no. 5174; all from Cell Signaling Technology, Inc.). .. The membranes were subsequently washed for 2 h with TBST (10 mM Tris, pH 7.5, 150 mM NaCl, and 0.05% Tween-20), and incubated with horseradish peroxidase-conjugated goat anti-rabbit secondary antibody (1:500; cat. no. sc-2004; Santa Cruz Biotechnology, Inc.) for 1 h at room temperature.

    Article Title: Anterior thalamic nuclei deep brain stimulation inhibits mossy fiber sprouting via 3′,5′-cyclic adenosine monophosphate/protein kinase A signaling pathway in a chronic epileptic monkey model
    Article Snippet: Total protein (60 μg) was resolved by SDS-polyacrylamide gel electrophoresis on a 12% polyacrylamide gel and transferred to polyvinylidene difluoride membrane (Millipore, Billerica, MA, USA). .. The membranes were blocked with 10% milk for 1 h and then incubated with the following primary antibodies: rabbit anti-β-actin (1:5000; Sigma-Aldrich, St. Louis, MO, USA, A5060), rabbit anti-phosphorylated (p)-Akt (1:1000, 4060S; Cell Signaling Technology, Danvers, MA, USA), rabbit anti-Akt (1:1000, 4691S; Cell Signaling Technology, Danvers, MA, USA), and rabbit anti-PKA (1:1000, LS-C63197-50; Lifespan Biosciences, Seattle, WA, USA). .. The membranes were then incubated with the secondary antibody and the protein bands were visualized with enhanced chemiluminescence and quantified using ImageJ software.

    other:

    Article Title: miR-126-5p regulates H9c2 cell proliferation and apoptosis under hypoxic conditions by targeting IL-17A
    Article Snippet: Proteins (10 μg/lane) were separated via 10% SDS-PAGE (cat. no. P1200-25T; Beijing Solarbio Science & Technology Co., Ltd.) and transferred to PVDF membranes (Beijing Solarbio Science & Technology Co., Ltd.).

    Article Title: Knockdown of ETV4 promotes autophagy-dependent apoptosis in GBM cells by reducing the transcriptional activation of EMP1
    Article Snippet: Protein bands were visualized using SuperSignal West Pico Plus (Thermo Fisher Scientific, Inc.) and a GS800 densitometer scanner (Bio-Rad Laboratories, Inc.).

    Cell-Signaling:

    Article Title: Ginkgolide B inhibits hydrogen peroxide-induced apoptosis and attenuates cytotoxicity via activating the PI3K/Akt/mTOR signaling pathway in H9c2 cells
    Article Snippet: .. The membranes were blocked with 5% BSA (Gibco; Thermo Fisher Scientific, Inc.) at room temperature for 2 h and incubated overnight at 4°C with primary antibodies against rabbit anti-Bax (1:1,000; cat. no. 2772), rabbit anti-Bcl-2 (1:1,000; cat. no. 2870), rabbit anti-cleaved caspase-3 (1:1,000; cat. no. 9664), rabbit anti-caspase-3 (1:1,000; cat. no. 9662), rabbit anti-Akt (1:1,000; cat. no. 9272), rabbit anti-phosphorylated (p)-Akt (p-Akt; 1:1,000; cat. no. 4060), rabbit anti-mTOR (1:1,000; cat. no. 2983), rabbit anti-p-mTOR (p-mTOR; 1:1,000; cat. no. 5536), and rabbit anti-GAPDH (1:1,000; cat. no. 5174; all from Cell Signaling Technology, Inc.). .. The membranes were subsequently washed for 2 h with TBST (10 mM Tris, pH 7.5, 150 mM NaCl, and 0.05% Tween-20), and incubated with horseradish peroxidase-conjugated goat anti-rabbit secondary antibody (1:500; cat. no. sc-2004; Santa Cruz Biotechnology, Inc.) for 1 h at room temperature.

    Article Title: Anterior thalamic nuclei deep brain stimulation inhibits mossy fiber sprouting via 3′,5′-cyclic adenosine monophosphate/protein kinase A signaling pathway in a chronic epileptic monkey model
    Article Snippet: Total protein (60 μg) was resolved by SDS-polyacrylamide gel electrophoresis on a 12% polyacrylamide gel and transferred to polyvinylidene difluoride membrane (Millipore, Billerica, MA, USA). .. The membranes were blocked with 10% milk for 1 h and then incubated with the following primary antibodies: rabbit anti-β-actin (1:5000; Sigma-Aldrich, St. Louis, MO, USA, A5060), rabbit anti-phosphorylated (p)-Akt (1:1000, 4060S; Cell Signaling Technology, Danvers, MA, USA), rabbit anti-Akt (1:1000, 4691S; Cell Signaling Technology, Danvers, MA, USA), and rabbit anti-PKA (1:1000, LS-C63197-50; Lifespan Biosciences, Seattle, WA, USA). .. The membranes were then incubated with the secondary antibody and the protein bands were visualized with enhanced chemiluminescence and quantified using ImageJ software.



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    Effects of OR on protein expression of PI3K, Akt, <t>p‐Akt,</t> and PTEN proteins in the ovary of rats. (a) Indicated representative immunoblots of PI3K, Akt, p‐Akt, and PTEN proteins in the ovaries of each group. (b) Shows the expression of PI3K protein relative to GAPDH protein. (c) Shows the expression of Akt protein relative to GAPDH protein. (d) Shows the expression of p‐Akt protein relative to GAPDH protein. (e) Shows the expression of p‐Akt protein relative to Akt protein. (f) Shows the expression of PTEN protein relative to GAPDH protein. C, control group; M, model group; ORH, high‐dose OR group; ORL, low‐dose OR group; P, positive drug group. Data are represented as the mean ± SD from 3 animals in each group. * p < 0.05 and ** p < 0.01 versus M group, # p < 0.05 and ## p < 0.01 versus P group. Full uncropped blots for this panel are presented in Supplementary File S2 (Figure ).
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    Effects of OR on protein expression of PI3K, Akt, <t>p‐Akt,</t> and PTEN proteins in the ovary of rats. (a) Indicated representative immunoblots of PI3K, Akt, p‐Akt, and PTEN proteins in the ovaries of each group. (b) Shows the expression of PI3K protein relative to GAPDH protein. (c) Shows the expression of Akt protein relative to GAPDH protein. (d) Shows the expression of p‐Akt protein relative to GAPDH protein. (e) Shows the expression of p‐Akt protein relative to Akt protein. (f) Shows the expression of PTEN protein relative to GAPDH protein. C, control group; M, model group; ORH, high‐dose OR group; ORL, low‐dose OR group; P, positive drug group. Data are represented as the mean ± SD from 3 animals in each group. * p < 0.05 and ** p < 0.01 versus M group, # p < 0.05 and ## p < 0.01 versus P group. Full uncropped blots for this panel are presented in Supplementary File S2 (Figure ).
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    Effects of OR on protein expression of PI3K, Akt, <t>p‐Akt,</t> and PTEN proteins in the ovary of rats. (a) Indicated representative immunoblots of PI3K, Akt, p‐Akt, and PTEN proteins in the ovaries of each group. (b) Shows the expression of PI3K protein relative to GAPDH protein. (c) Shows the expression of Akt protein relative to GAPDH protein. (d) Shows the expression of p‐Akt protein relative to GAPDH protein. (e) Shows the expression of p‐Akt protein relative to Akt protein. (f) Shows the expression of PTEN protein relative to GAPDH protein. C, control group; M, model group; ORH, high‐dose OR group; ORL, low‐dose OR group; P, positive drug group. Data are represented as the mean ± SD from 3 animals in each group. * p < 0.05 and ** p < 0.01 versus M group, # p < 0.05 and ## p < 0.01 versus P group. Full uncropped blots for this panel are presented in Supplementary File S2 (Figure ).
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    KSM inhibits CHI3L1-stimulated Erk and <t>Akt</t> activation and the antiapoptotic effect of CHI3L1 in macrophages. ( A ) AMJ-C11 mouse lung macrophages were stimulated with recombinant (r) CHI3L1 overnight, and the activation of Erk and Akt was detected by Western blot evaluations. pErk, <t>phosphorylated</t> Erk; T-Erk, total Erk; pAkt, phosphorylated Akt, T-Akt, total Akt. Right panel, densitometric quantitation on the blots of pErk and pAkt. ( B ) After exposure of AMJ-C11 cells with H 2 O 2 (1 mM, 24 hours) with and without rCHI3L1 and KSM treatment, cellular apoptosis (% of Annexin V+ cells) was measured by FACS evaluations. ( A and B ) are representatives of 3 independent experiments. Right panel, the values of Annexin 5 (+) cells (%) are the mean ± SEM. * P <0.05, ** P <0.01, *** P <0.001 (One-Way ANOVA, multiple comparisons).
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    Cell Signaling Technology Inc rabbit anti phosphorylated akt p akt
    KSM inhibits CHI3L1-stimulated Erk and <t>Akt</t> activation and the antiapoptotic effect of CHI3L1 in macrophages. ( A ) AMJ-C11 mouse lung macrophages were stimulated with recombinant (r) CHI3L1 overnight, and the activation of Erk and Akt was detected by Western blot evaluations. pErk, <t>phosphorylated</t> Erk; T-Erk, total Erk; pAkt, phosphorylated Akt, T-Akt, total Akt. Right panel, densitometric quantitation on the blots of pErk and pAkt. ( B ) After exposure of AMJ-C11 cells with H 2 O 2 (1 mM, 24 hours) with and without rCHI3L1 and KSM treatment, cellular apoptosis (% of Annexin V+ cells) was measured by FACS evaluations. ( A and B ) are representatives of 3 independent experiments. Right panel, the values of Annexin 5 (+) cells (%) are the mean ± SEM. * P <0.05, ** P <0.01, *** P <0.001 (One-Way ANOVA, multiple comparisons).
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    KSM inhibits CHI3L1-stimulated Erk and <t>Akt</t> activation and the antiapoptotic effect of CHI3L1 in macrophages. ( A ) AMJ-C11 mouse lung macrophages were stimulated with recombinant (r) CHI3L1 overnight, and the activation of Erk and Akt was detected by Western blot evaluations. pErk, <t>phosphorylated</t> Erk; T-Erk, total Erk; pAkt, phosphorylated Akt, T-Akt, total Akt. Right panel, densitometric quantitation on the blots of pErk and pAkt. ( B ) After exposure of AMJ-C11 cells with H 2 O 2 (1 mM, 24 hours) with and without rCHI3L1 and KSM treatment, cellular apoptosis (% of Annexin V+ cells) was measured by FACS evaluations. ( A and B ) are representatives of 3 independent experiments. Right panel, the values of Annexin 5 (+) cells (%) are the mean ± SEM. * P <0.05, ** P <0.01, *** P <0.001 (One-Way ANOVA, multiple comparisons).
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    Image Search Results


    Effects of OR on protein expression of PI3K, Akt, p‐Akt, and PTEN proteins in the ovary of rats. (a) Indicated representative immunoblots of PI3K, Akt, p‐Akt, and PTEN proteins in the ovaries of each group. (b) Shows the expression of PI3K protein relative to GAPDH protein. (c) Shows the expression of Akt protein relative to GAPDH protein. (d) Shows the expression of p‐Akt protein relative to GAPDH protein. (e) Shows the expression of p‐Akt protein relative to Akt protein. (f) Shows the expression of PTEN protein relative to GAPDH protein. C, control group; M, model group; ORH, high‐dose OR group; ORL, low‐dose OR group; P, positive drug group. Data are represented as the mean ± SD from 3 animals in each group. * p < 0.05 and ** p < 0.01 versus M group, # p < 0.05 and ## p < 0.01 versus P group. Full uncropped blots for this panel are presented in Supplementary File S2 (Figure ).

    Journal: Food Science & Nutrition

    Article Title: Oviductus Ranae as a Functional Food for Ovary Protection: Ameliorating Premature Ovarian Failure and Modulating PI3K /Akt and Apoptosis Pathways in Rats

    doi: 10.1002/fsn3.72089

    Figure Lengend Snippet: Effects of OR on protein expression of PI3K, Akt, p‐Akt, and PTEN proteins in the ovary of rats. (a) Indicated representative immunoblots of PI3K, Akt, p‐Akt, and PTEN proteins in the ovaries of each group. (b) Shows the expression of PI3K protein relative to GAPDH protein. (c) Shows the expression of Akt protein relative to GAPDH protein. (d) Shows the expression of p‐Akt protein relative to GAPDH protein. (e) Shows the expression of p‐Akt protein relative to Akt protein. (f) Shows the expression of PTEN protein relative to GAPDH protein. C, control group; M, model group; ORH, high‐dose OR group; ORL, low‐dose OR group; P, positive drug group. Data are represented as the mean ± SD from 3 animals in each group. * p < 0.05 and ** p < 0.01 versus M group, # p < 0.05 and ## p < 0.01 versus P group. Full uncropped blots for this panel are presented in Supplementary File S2 (Figure ).

    Article Snippet: The extract was separated by SDS‐PAGE gel electrophoresis (Beijing Dingguo Changsheng Biotechnology Co. Ltd., Beijing, China) and transferred to PVDF membrane (Millipore, USA), blocked with TBST buffer containing 5% BSA for 20 min, and 3 mL of the corresponding antibody PI3K (PI3 Kinase p110 delta Rabbit mAb, ABclonal Technology, Wuhan, China), Akt (AKT1 Rabbit mAb, ABclonal Technology, Wuhan, China), phosphorylated Akt (p‐Akt) (Phospho‐Akt‐S473 Rabbit mAb, ABclonal Technology, Wuhan, China), phosphatase and tensin homolog deleted on chromosome 10 (PTEN) (PTEN Bcl‐2 Rabbit mAb , ABclonal Technology, Wuhan, China), Bcl2 ( Bcl‐2 Rabbit mAb , ABclonal Technology, Wuhan, China), TBST dilutions (1:1000) of Bax (Bax Rabbit mAb, ABclonal Technology, Wuhan, China), GAPDH ( GAPDH Rabbit mAb , ABclonal Technology, Wuhan, China), incubated at room temperature for 20 min, and after TBST membrane washing, 3 mL of secondary antibody (CST Company, Fig. USA) TBST dilute solution (1:2000), incubate at room temperature for 20 min, wash the TBST membrane and soak the PVDF membrane into TBST solution to prepare E CL chemiluminescence solution (Millipore, USA), drop it on the target protein strip of PVDF membrane, incubate at room temperature for 2 min in the dark, Images of the bands were collected using a chemiluminescence system (Tanon 4600, Shanghai Tianneng Technology Co. Ltd., Shanghai, China) and the gray values of the bands were quantified using Image J software.

    Techniques: Expressing, Western Blot, Control

    KSM inhibits CHI3L1-stimulated Erk and Akt activation and the antiapoptotic effect of CHI3L1 in macrophages. ( A ) AMJ-C11 mouse lung macrophages were stimulated with recombinant (r) CHI3L1 overnight, and the activation of Erk and Akt was detected by Western blot evaluations. pErk, phosphorylated Erk; T-Erk, total Erk; pAkt, phosphorylated Akt, T-Akt, total Akt. Right panel, densitometric quantitation on the blots of pErk and pAkt. ( B ) After exposure of AMJ-C11 cells with H 2 O 2 (1 mM, 24 hours) with and without rCHI3L1 and KSM treatment, cellular apoptosis (% of Annexin V+ cells) was measured by FACS evaluations. ( A and B ) are representatives of 3 independent experiments. Right panel, the values of Annexin 5 (+) cells (%) are the mean ± SEM. * P <0.05, ** P <0.01, *** P <0.001 (One-Way ANOVA, multiple comparisons).

    Journal: ImmunoTargets and Therapy

    Article Title: Kasugamycin Inhibits Melanoma Lung Metastasis and CHI3L1-Driven M2-Like Tumor-Associated Macrophage Differentiation

    doi: 10.2147/ITT.S563951

    Figure Lengend Snippet: KSM inhibits CHI3L1-stimulated Erk and Akt activation and the antiapoptotic effect of CHI3L1 in macrophages. ( A ) AMJ-C11 mouse lung macrophages were stimulated with recombinant (r) CHI3L1 overnight, and the activation of Erk and Akt was detected by Western blot evaluations. pErk, phosphorylated Erk; T-Erk, total Erk; pAkt, phosphorylated Akt, T-Akt, total Akt. Right panel, densitometric quantitation on the blots of pErk and pAkt. ( B ) After exposure of AMJ-C11 cells with H 2 O 2 (1 mM, 24 hours) with and without rCHI3L1 and KSM treatment, cellular apoptosis (% of Annexin V+ cells) was measured by FACS evaluations. ( A and B ) are representatives of 3 independent experiments. Right panel, the values of Annexin 5 (+) cells (%) are the mean ± SEM. * P <0.05, ** P <0.01, *** P <0.001 (One-Way ANOVA, multiple comparisons).

    Article Snippet: Protein lysates (25 μg) from lung tissues or cells were subjected to SDS-PAGE, transferred to membranes, and immunoblotted with primary antibodies against CD163 (#PA5-109327, Thermo Fisher Scientific), CD206 (E6T5J, #24595S, Cell Signaling Technology), phosphorylated AKT (p-Akt) (193H12, #4058S, Cell Signaling Technology), and total Akt (11E7, #4685S, Cell Signaling Technology), phosphorylated EGFR (Tyr1068) (p-EGFR) (#44-788G, Thermo Fisher Scientific), EGFR (D38B1, #4267S, Cell Signaling Technology), phosphorylated Erk (p-Erk) (#9101S, Cell Signaling Technology), total Erk (#9102S, Cell Signaling Technology), phosphorylated STAT3 (Tyr705) (p-STAT3) (D3A7, #9145, Cell Signaling Technology), total STAT3 (79D7, #4904, Cell Signaling Technology) and b-actin (C4, #sc-47778 HRP, Santa Cruz Biotechnology).

    Techniques: Activation Assay, Recombinant, Western Blot, Quantitation Assay